Under this page you can find protocols, working information for the group, among other information. Most of this is only relevant for members of the lab.
Lab meeting
Link to schedule for weekly lab meetings
Lab wiki
Code
Code for newer papers can be found on my github page or on the github of the lead author, typically. For older papers, please contact me.
Protocols
Below are a number of protocols I’ve used, modified, and tried to write up in a clear manner. Feel free to contact me with any questions.
RNA extractions for copepods snap frozen in water: link to come
DNA Extraction
A dirt cheap DNA extraction. About $0.70/sample. Gives great yield, high MW, and only takes ~2 hours (after digestion). I’ve never had this fail, but have only ever tested it with fin clips or whole fry.
RAD-seq library preps
BestRAD library prep
This is modified from a protocol developed by Mike Miller’s lab at UCD. Ali, Omar A., et al. “RAD capture (Rapture): flexible and efficient sequence-based genotyping.” Genetics 202.2 (2016): 389-400.
There have been a number of iterations of this protocol. If the link below seems out of date, I probably have a newer one that might be a bit more thorough.